› 1qw9

X-ray diffraction
1.2Å resolution

Crystal structure of a family 51 alpha-L-arabinofuranosidase in complex with 4-nitrophenyl-Ara

Released:
DOI: 10.2210/pdb1qw9/pdb
PDB entry 1qw9 coloured by chain, front view.
PDB entry 1qw9 coloured by chain, side view.
PDB entry 1qw9 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure and snapshots along the reaction pathway of a family 51 alpha-L-arabinofuranosidase.
Hövel K, Shallom D, Niefind K, Belakhov V, Shoham G, Baasov T, Shoham Y, Schomburg D
EMBO J 22 4922-32 (2003)
PMID: 14517232

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intracellular exo-alpha-(1->5)-L-arabinofuranosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 502 amino acids
Theoretical weight: 57.23 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XBQ3 (Residues: 1-502; Coverage: 100%)
Gene name: abfA
Sequence domains: Alpha-L-arabinofuranosidase C-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand KHP 2 x KHP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: R3
Unit cell:
a: 179.311Å b: 179.311Å c: 100.397Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.16 0.179
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Thermostable enzymes as biocatalysts in the biofuel industry.
Yeoman et al. (2010)
6 more

11 mentions without citation

Crystallographic model validation: from diagnosis to healing.
Richardson et al. (2013)
10 more