› 1qxp

X-ray diffraction
2.8Å resolution

Crystal Structure of a mu-like calpain

Released:
DOI: 10.2210/pdb1qxp/pdb
PDB entry 1qxp coloured by chain, front view.
PDB entry 1qxp coloured by chain, side view.
PDB entry 1qxp coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of a micro-like calpain reveals a partially activated conformation with low Ca2+ requirement.
Pal GP, De Veyra T, Elce JS, Jia Z
Structure 11 1521-6 (2003)
PMID: 14656436

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calpain small subunit 1; Calpain-1 catalytic subunit; Calpain-2 catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 900 amino acids
Theoretical weight: 103.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07009 (Residues: 1-49, 639-700; Coverage: 16%)
  • Canonical: P97571 (Residues: 60-650; Coverage: 83%)
  • Canonical: Q64537 (Residues: 88-270; Coverage: 68%)
Gene names: Capn1, Capn2, Capn4, Capns1, Cls1, Css1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 72.738Å b: 184.596Å c: 86.37Å
α: 90° β: 100.74° γ: 90°
R-values:
R R work R free
0.233 0.229 0.311
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The calpains: modular designs and functional diversity.
Croall et al. (2007)
4 more

7 mentions without citation

Small-angle X-ray scattering of calpain-5 reveals a highly open conformation among calpains.
Gakhar et al. (2016)
6 more