› 1qxt

X-ray diffraction
2Å resolution

Crystal structure of precyclized intermediate for the green fluorescent protein R96A variant (A)

Released:
DOI: 10.2210/pdb1qxt/pdb
PDB entry 1qxt coloured by chain, front view.
PDB entry 1qxt coloured by chain, side view.
PDB entry 1qxt coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
Barondeau DP, Putnam CD, Kassmann CJ, Tainer JA, Getzoff ED
Proc Natl Acad Sci U S A 100 12111-6 (2003)
PMID: 14523232

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 25.69 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-229; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 51.928Å b: 61.571Å c: 69.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.257
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
23 more

5 mentions without citation

Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
Barondeau et al. (2003)
4 more