› 1qz0

X-ray diffraction
1.5Å resolution

Crystal Structure of the Yersinia Pestis Phosphatase YopH in Complex with a Phosphotyrosyl Mimetic-Containing Hexapeptide

Released:
DOI: 10.2210/pdb1qz0/pdb
PDB entry 1qz0 coloured by chain, front view.
PDB entry 1qz0 coloured by chain, side view.
PDB entry 1qz0 coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
High-resolution structure of the Yersinia pestis protein tyrosine phosphatase YopH in complex with a phosphotyrosyl mimetic-containing hexapeptide.
Phan J, Lee K, Cherry S, Tropea JE, Burke TR, Waugh DS
Biochemistry 42 13113-21 (2003)
PMID: 14609321

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130630 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
protein-tyrosine-phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 33.55 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O68720 (Residues: 164-468; Coverage: 65%)
Gene names: Y0013, YPCD1.67c, yopH
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
ASP-ALA-ASP-GLU-FTY-LEU-NH2 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 7 amino acids
Theoretical weight: 837 Da
Source organism: Yersinia pestis
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand FTY 4 x FTY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 47.292Å b: 53.45Å c: 69.066Å
α: 109.61° β: 104.75° γ: 89.98°
R-values:
R R work R free
0.188 0.188 0.209
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

Yersinia outer proteins: Yops.
Trosky et al. (2008)
8 more

9 mentions without citation

Structural analysis of inhibition mechanisms of aurintricarboxylic acid on SARS-CoV polymerase and other proteins.
Yap et al. (2005)
8 more