› 1qzw

X-ray diffraction
4.1Å resolution

Crystal structure of the complete core of archaeal SRP and implications for inter-domain communication

Released:
DOI: 10.2210/pdb1qzw/pdb
PDB entry 1qzw coloured by chain, front view.
PDB entry 1qzw coloured by chain, side view.
PDB entry 1qzw coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Crystal structure of the complete core of archaeal signal recognition particle and implications for interdomain communication.
Rosendal KR, Wild K, Montoya G, Sinning I
Proc Natl Acad Sci U S A 100 14701-6 (2003)
PMID: 14657338

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189074 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Signal recognition particle 54 kDa protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 440 amino acids
Theoretical weight: 49.46 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZE7 (Residues: 1-432; Coverage: 97%)
Gene names: SSO0971, srp54
Sequence domains:
7S RNA Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 47 nucleotides
Theoretical weight: 15.56 KDa
Source organism: Saccharolobus solfataricus
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand GTP 4 x GTP
Ligand CCC 4 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P31
Unit cell:
a: 137.763Å b: 137.763Å c: 307.894Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.34 0.34 0.387
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Delivering proteins for export from the cytosol.
Cross et al. (2009)
15 more

15 mentions without citation

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
14 more