Function and Biology Details
Reaction catalysed:
PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- tRNA intron endonuclease, catalytic domain-like
- tRNA-splicing endonuclease
- tRNA intron endonuclease, catalytic domain-like superfamily
- tRNA intron endonuclease, N-terminal domain superfamily
- tRNA endonuclease-like domain superfamily
- tRNA intron endonuclease, N-terminal
- tRNA-splicing endonuclease, archaeal long subfamily
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
tRNA-splicing endonuclease (preferred)
PDBe Complex ID:
PDB-CPX-128117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA-splicing endonuclease
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 36 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 305 amino acids
Theoretical weight: 36 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
- Canonical:
O29362 (Residues: 1-305; Coverage: 100%)
Sequence domains:
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 5.0.2
Spacegroup:
P21212
Expression system: Escherichia coli
