1r1k

X-ray diffraction
2.9Å resolution

Crystal structure of the ligand-binding domains of the heterodimer EcR/USP bound to ponasterone A

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
PDBe Complex ID:
PDB-CPX-127580 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ecdysone receptor Chain: D
Molecule details ›
Chain: D
Length: 266 amino acids
Theoretical weight: 30.37 KDa
Source organism: Heliothis virescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O18473 (Residues: 298-342, 343-550; Coverage: 44%)
Gene names: EcR, NR1H1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
NR LBD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.95 KDa
Source organism: Heliothis virescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7SIF6 (Residues: 3-264; Coverage: 99%)
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P3121
Unit cell:
a: 57.401Å b: 57.401Å c: 302.696Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.243 0.304
Expression system: Escherichia coli BL21(DE3)