› 1r1v

X-ray diffraction
2.3Å resolution

Crystal structure of the metal-sensing transcriptional repressor CzrA from Staphylococcus aureus in the Zn2-form

Released:
DOI: 10.2210/pdb1r1v/pdb
PDB entry 1r1v coloured by chain, front view.
PDB entry 1r1v coloured by chain, side view.
PDB entry 1r1v coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins.
Eicken C, Pennella MA, Chen X, Koshlap KM, VanZile ML, Sacchettini JC, Giedroc DP
J Mol Biol 333 683-95 (2003)
PMID: 14568530

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-131433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH arsR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.01 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O85142 (Residues: 1-106; Coverage: 100%)
Gene names: A6760_11845, BN1321_350009, CV021_10345, CzrA, EP54_06885, EQ90_13065, FAF17_13815, G6W63_05680, G6Y24_05035, GO793_16165, GO814_05535, GO941_16585, GO942_06145, GQX37_08730, GZ156_05085, GZ163_08250, H2639_10805, HMPREF3211_00009, M1K003_2263, NCTC10702_03381, NCTC13131_01480, NCTC5664_01801, NCTC6133_02909, NCTC7878_02904, NCTC7972_02574, QU38_06130, SAMEA2078260_01939, SAMEA2078588_01910, SAMEA2080344_01776, SAMEA2081063_01791, SAMEA4008575_00680, SAMEA70146418_02191, SAMEA70153168_02326, SAMEA70245418_02324, czrA, czrA_1, czrA_2, rzcA
Sequence domains: Bacterial regulatory protein, arsR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 54.444Å b: 75.37Å c: 50.625Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.195 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
14 more

11 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
10 more