› 1r3c

X-ray diffraction
2Å resolution

THE STRUCTURE OF P38ALPHA C162S MUTANT

Released:
DOI: 10.2210/pdb1r3c/pdb
PDB entry 1r3c coloured by chain, front view.
PDB entry 1r3c coloured by chain, side view.
PDB entry 1r3c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lattice stabilization and enhanced diffraction in human p38 alpha crystals by protein engineering.
Patel SB, Cameron PM, Frantz-Wattley B, O'Neill E, Becker JW, Scapin G
Biochim Biophys Acta 1696 67-73 (2004)
PMID: 14726206

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 14 Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 41.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16539 (Residues: 1-360; Coverage: 100%)
Gene names: CSBP, CSBP1, CSBP2, CSPB1, MAPK14, MXI2, SAPK2A
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 45.162Å b: 84.799Å c: 124.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Fragment-based screening by protein crystallography: successes and pitfalls.
Chilingaryan et al. (2012)
11 other articles

5 mentions without citation

p38α Mitogen-Activated Protein Kinase Is a Druggable Target in Pancreatic Adenocarcinoma.
Yang et al. (2019)
4 more