› 1r3d

X-ray diffraction
1.9Å resolution

Crystal structure of protein VC1974 from Vibrio cholerae, Pfam abhydrolase

Released:
DOI: 10.2210/pdb1r3d/pdb
PDB entry 1r3d coloured by chain, front view.
PDB entry 1r3d coloured by chain, side view.
PDB entry 1r3d coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Gorman J, Shapiro L, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.26 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KQM4 (Residues: 2-263; Coverage: 100%)
Gene names: VC_1974, menH
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P42212
Unit cell:
a: 99.47Å b: 99.47Å c: 53.492Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.216
Expression system: Escherichia coli BL21

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