› 1r4r

X-ray diffraction
3Å resolution

Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA

Released:
DOI: 10.2210/pdb1r4r/pdb
PDB entry 1r4r coloured by chain, front view.
PDB entry 1r4r coloured by chain, side view.
PDB entry 1r4r coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA.
Luisi BF, Xu WX, Otwinowski Z, Freedman LP, Yamamoto KR, Sigler PB
Nature 352 497-505 (1991)
PMID: 1865905

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114499 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Glucocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 10.23 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06536 (Residues: 440-525; Coverage: 11%)
Gene names: Grl, Nr3c1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*TP*CP*AP*GP*AP*AP*CP*AP*TP*GP*AP*TP*GP*TP*TP*CP*TP*CP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
5'-D(*CP*TP*GP*AP*GP*AP*AP*CP*AP*TP*CP*AP*TP*GP*TP*TP*CP*TP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Rattus norvegicus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 38.5Å b: 99.7Å c: 121.9Å
α: 90° β: 90° γ: 90°
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

218 review citations

The RXR heterodimers and orphan receptors.
Mangelsdorf et al. (1995)
217 more

18 mentions without citation

Glucocorticoid receptor control of transcription: precision and plasticity via allostery.
Weikum et al. (2017)
17 more