› 1r6u

X-ray diffraction
2Å resolution

Crystal structure of an active fragment of human tryptophanyl-tRNA synthetase with cytokine activity

Released:
DOI: 10.2210/pdb1r6u/pdb
PDB entry 1r6u coloured by chain, front view.
PDB entry 1r6u coloured by chain, side view.
PDB entry 1r6u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional and crystal structure analysis of active site adaptations of a potent anti-angiogenic human tRNA synthetase.
Yang XL, Guo M, Kapoor M, Ewalt KL, Otero FJ, Skene RJ, McRee DE, Schimmel P
Structure 15 793-805 (2007)
PMID: 17637340

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149944 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 50.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23381 (Residues: 48-471; Coverage: 90%)
Gene names: IFI53, WARS, WARS1, WRS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TYM 1 x TYM
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 134.658Å b: 96.46Å c: 97.132Å
α: 90° β: 129.9° γ: 90°
R-values:
R R work R free
0.255 0.254 0.298
Expression system: Escherichia coli

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Citations

5 review citations

A survey of the year 2007 literature on applications of isothermal titration calorimetry.
Bjelić et al. (2008)
4 more

6 mentions without citation

Functional and crystal structure analysis of active site adaptations of a potent anti-angiogenic human tRNA synthetase.
Yang et al. (2007)
5 more