› 1r76

X-ray diffraction
2.65Å resolution

Structure of a pectate lyase from Azospirillum irakense

Released:
DOI: 10.2210/pdb1r76/pdb
PDB entry 1r76 coloured by chain, front view.
PDB entry 1r76 coloured by chain, side view.
PDB entry 1r76 coloured by chain, top view.
Source organism: Niveispirillum irakense
Primary publication:
Azospirillum irakense pectate lyase displays a toroidal fold.
Novoa De Armas H, Verboven C, De Ranter C, Desair J, Vande Broek A, Vanderleyden J, Rabijns A
Acta Crystallogr D Biol Crystallogr 60 999-1007 (2004)
PMID: 15159558

Function and Biology Details

Reaction catalysed: 
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectate lyase Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 44.55 KDa
Source organism: Niveispirillum irakense
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X592 (Residues: 25-432; Coverage: 100%)
Gene name: pelA
Sequence domains: Pectic acid lyase
Structure domains: Glycosyltransferase

Ligands and Environments

4 bound ligands:
Ligand HG 1 x HG
Ligand CL 1 x CL
Ligand IPA 1 x IPA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P6522
Unit cell:
a: 85.374Å b: 85.374Å c: 231.317Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.262
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial pectate lyases, structural and functional diversity.
Hugouvieux-Cotte-Pattat et al. (2014)
3 other articles