› 1r86

X-ray diffraction
1.8Å resolution

Crystal structure of the extracellular xylanase from Geobacillus stearothermophilus T-6 (XT6, monoclinic form): The E159A/E265A mutant at 1.8A resolution

Released:
DOI: 10.2210/pdb1r86/pdb
PDB entry 1r86 coloured by chain, front view.
PDB entry 1r86 coloured by chain, side view.
PDB entry 1r86 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Bar M, Golan G, Zolotnitsky G, Shoham Y, Shoham G

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 43.76 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P40943 (Residues: 29-407; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand ZN 7 x ZN
Ligand CL 2 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 121.244Å b: 61.251Å c: 89.463Å
α: 90° β: 119.28° γ: 90°
R-values:
R R work R free
0.181 0.181 0.215
Expression system: Escherichia coli BL21

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