› 1ra0

X-ray diffraction
1.12Å resolution

Bacterial cytosine deaminase D314G mutant bound to 5-fluoro-4-(S)-hydroxy-3,4-dihydropyrimidine.

Released:
DOI: 10.2210/pdb1ra0/pdb
PDB entry 1ra0 coloured by chain, front view.
PDB entry 1ra0 coloured by chain, side view.
PDB entry 1ra0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Random mutagenesis and selection of Escherichia coli cytosine deaminase for cancer gene therapy.
Mahan SD, Ireton GC, Knoeber C, Stoddard BL, Black ME
Protein Eng Des Sel 17 625-33 (2004)
PMID: 15381761

Function and Biology Details

Reaction catalysed: 
Cytosine + H(2)O = uracil + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine deaminase Chain: A
Molecule details ›
Chain: A
Length: 430 amino acids
Theoretical weight: 47.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25524 (Residues: 2-427; Coverage: 100%)
Gene names: JW0328, b0337, codA
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand FPY 1 x FPY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R32
Unit cell:
a: 109.257Å b: 109.257Å c: 240.842Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.168 0.172
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Nucleic acid delivery with chitosan and its derivatives.
Lai et al. (2009)
8 more

7 mentions without citation

The VSGB 2.0 model: a next generation energy model for high resolution protein structure modeling.
Li et al. (2011)
6 more