› 1rd5

X-ray diffraction
2.02Å resolution

Crystal structure of Tryptophan synthase alpha chain homolog BX1: a member of the chemical plant defense system

Released:
DOI: 10.2210/pdb1rd5/pdb
PDB entry 1rd5 coloured by chain, front view.
PDB entry 1rd5 coloured by chain, side view.
PDB entry 1rd5 coloured by chain, top view.
Source organism: Zea mays
Primary publication:
On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and BX1 from maize, two evolutionarily related enzymes.
Kulik V, Hartmann E, Weyand M, Frey M, Gierl A, Niks D, Dunn MF, Schlichting I
J Mol Biol 352 608-20 (2005)
PMID: 16120446

Function and Biology Details

Reactions catalysed: 
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-154639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole-3-glycerol phosphate lyase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.03 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P42390 (Residues: 87-346; Coverage: 75%)
Gene name: BX1
Sequence domains: Tryptophan synthase alpha chain
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: F222
Unit cell:
a: 91.094Å b: 159.815Å c: 162.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.299
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Benzoxazinoid biosynthesis, a model for evolution of secondary metabolic pathways in plants.
Frey et al. (2009)
4 more

3 mentions without citation

Functional classification of protein structures by local structure matching in graph representation.
Mills et al. (2018)
2 more