› 1rhc

X-ray diffraction
1.8Å resolution

F420-dependent secondary alcohol dehydrogenase in complex with an F420-acetone adduct

Released:
DOI: 10.2210/pdb1rhc/pdb
PDB entry 1rhc coloured by chain, front view.
PDB entry 1rhc coloured by chain, side view.
PDB entry 1rhc coloured by chain, top view.
Source organism: Methanoculleus thermophilus
Primary publication:
Coenzyme binding in F420-dependent secondary alcohol dehydrogenase, a member of the bacterial luciferase family.
Aufhammer SW, Warkentin E, Berk H, Shima S, Thauer RK, Ermler U
Structure 12 361-70 (2004)
PMID: 15016352

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131686 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 37.2 KDa
Source organism: Methanoculleus thermophilus
UniProt:
  • Canonical: O93734 (Residues: 1-330; Coverage: 100%)
Gene name: adf
Sequence domains: Luciferase-like monooxygenase
Structure domains: Luciferase-like domain

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand K 1 x K
Ligand F42 1 x F42
Ligand ACN 1 x ACN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2221
Unit cell:
a: 86.824Å b: 156.316Å c: 60.199Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.188

Quick links

Citations

2 review citations

Bioinorganic insights of the PQQ-dependent alcohol dehydrogenases.
Sarmiento-Pavía et al. (2021)
1 more

11 mentions without citation

Physiology, Biochemistry, and Applications of F420- and Fo-Dependent Redox Reactions.
Greening et al. (2016)
10 more