› 1rit

X-ray diffraction
2.85Å resolution

Crystal structure of Peanut lectin in complex with meso-tetrasulphonatophenylporphyrin and lactose

Released:
DOI: 10.2210/pdb1rit/pdb
PDB entry 1rit coloured by chain, front view.
PDB entry 1rit coloured by chain, side view.
PDB entry 1rit coloured by chain, top view.
Source organism: Arachis hypogaea
Primary publication:
Crystal structures of the PNA-porphyrin complex in the presence and absence of lactose: mapping the conformational changes on lactose binding, interacting surfaces, and supramolecular aggregations.
Goel M, Damai RS, Sethi DK, Kaur KJ, Maiya BG, Swamy MJ, Salunke DM
Biochemistry 44 5588-96 (2005)
PMID: 15823017

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galactose-binding lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 25.21 KDa
Source organism: Arachis hypogaea
UniProt:
  • Canonical: P02872 (Residues: 24-259; Coverage: 94%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand SFP 11 x SFP
Ligand CA 4 x CA
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P32
Unit cell:
a: 94.9Å b: 94.9Å c: 144.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.235 0.26

Quick links

Citations

2 review citations

Beyond carbohydrate binding: new directions in plant lectin research.
Komath et al. (2006)
1 more

1 mention without citation

Binding of a Soluble meso-Tetraarylporphyrin to Human Galectin-7 Induces Oligomerization and Modulates Its Pro-Apoptotic Activity.
López de Los Santos et al. (2020)