› 1rj4

X-ray diffraction
2Å resolution

Structure of a Cell Wall Invertase Inhibitor from Tobacco in Complex with Cd2+

Released:
DOI: 10.2210/pdb1rj4/pdb
PDB entry 1rj4 coloured by chain, front view.
PDB entry 1rj4 coloured by chain, side view.
PDB entry 1rj4 coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension.
Hothorn M, D'Angelo I, Márquez JA, Greiner S, Scheffzek K
J Mol Biol 335 987-95 (2004)
PMID: 14698293

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectinesterase inhibitor domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 151 amino acids
Theoretical weight: 16.09 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli
UniProt:
  • Canonical: O49908 (Residues: 20-166; Coverage: 100%)
Gene name: LOC107808322
Sequence domains: Plant invertase/pectin methylesterase inhibitor
Structure domains: Invertase/pectin methylesterase inhibitor family protein

Ligands and Environments

2 bound ligands:
Ligand CD 8 x CD
Ligand BTB 4 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 59.17Å b: 95.639Å c: 126.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.255
Expression system: Escherichia coli

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Citations

3 review citations

Recent advances in plant cell wall proteomics.
Jamet et al. (2008)
2 more