› 1rjx

X-ray diffraction
2.3Å resolution

Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT

Released:
DOI: 10.2210/pdb1rjx/pdb
PDB entry 1rjx coloured by chain, front view.
PDB entry 1rjx coloured by chain, side view.
PDB entry 1rjx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterization of Lys-698-to-Met substitution in human plasminogen catalytic domain.
Terzyan S, Wakeham N, Zhai P, Rodgers K, Zhang XC
Proteins 56 277-84 (2004)
PMID: 15211511

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa, higher selectivity than trypsin. Converts fibrin into soluble products.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo 24-mer
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-133230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmin light chain B Chain: B
Molecule details ›
Chain: B
Length: 247 amino acids
Theoretical weight: 27.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00747 (Residues: 564-810; Coverage: 31%)
Gene name: PLG
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I432
Unit cell:
a: 158.396Å b: 158.396Å c: 158.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

The structure of thrombin, a chameleon-like proteinase.
Bode W. (2005)
1 more