› 1rlg

X-ray diffraction
2.7Å resolution

Molecular basis of Box C/D RNA-protein interaction: co-crystal structure of the Archaeal sRNP intiation complex

Released:
DOI: 10.2210/pdb1rlg/pdb
PDB entry 1rlg coloured by chain, front view.
PDB entry 1rlg coloured by chain, side view.
PDB entry 1rlg coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Molecular basis of box C/D RNA-protein interactions; cocrystal structure of archaeal L7Ae and a box C/D RNA.
Moore T, Zhang Y, Fenley MO, Li H
Structure 12 807-18 (2004)
PMID: 15130473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128133 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein eL8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.21 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O29494 (Residues: 1-119; Coverage: 100%)
Gene names: AF_0764, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
25-MER Chains: C, D
Molecule details ›
Chains: C, D
Length: 25 nucleotides
Theoretical weight: 8.33 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5BU 6 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P23
Unit cell:
a: 120.584Å b: 120.584Å c: 120.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.26
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

20 review citations

From selenium to selenoproteins: synthesis, identity, and their role in human health.
Papp et al. (2007)
19 more

25 mentions without citation

The structure and function of small nucleolar ribonucleoproteins.
Reichow et al. (2007)
24 more