› 1ro8

X-ray diffraction
2.05Å resolution

Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analogue, cytidine-5'-monophosphate

Released:
DOI: 10.2210/pdb1ro8/pdb
PDB entry 1ro8 coloured by chain, front view.
PDB entry 1ro8 coloured by chain, side view.
PDB entry 1ro8 coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog.
Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, Strynadka NC
Nat Struct Mol Biol 11 163-70 (2004)
PMID: 14730352

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192239 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-2,3/8-sialyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 30.94 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9LAK3 (Residues: 1-259; Coverage: 89%)
Gene name: cst-II
Sequence domains: Alpha-2,3-sialyltransferase (CST-I)
Structure domains: Alpha-2,3-sialyltransferase

Ligands and Environments

1 bound ligand:
Ligand C5P 2 x C5P
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4
Unit cell:
a: 115.434Å b: 115.434Å c: 41.059Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.22 0.262
Expression system: Escherichia coli BL21

Quick links

Citations

22 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
21 more

1 mention without citation

Structural and kinetic analysis of substrate binding to the sialyltransferase Cst-II from Campylobacter jejuni.
Lee et al. (2011)