› 1rov

X-ray diffraction
2Å resolution

Lipoxygenase-3 Treated with Cumene Hydroperoxide

Released:
DOI: 10.2210/pdb1rov/pdb
PDB entry 1rov coloured by chain, front view.
PDB entry 1rov coloured by chain, side view.
PDB entry 1rov coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Interaction between non-heme iron of lipoxygenases and cumene hydroperoxide: basis for enzyme activation, inactivation, and inhibition.
Vahedi-Faridi A, Brault PA, Shah P, Kim YW, Dunham WR, Funk MO
J Am Chem Soc 126 2006-15 (2004)
PMID: 14971933

Function and Biology Details

Reaction catalysed: 
Linoleate + O(2) = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 9S-lipoxygenase-3 Chain: A
Molecule details ›
Chain: A
Length: 857 amino acids
Theoretical weight: 96.95 KDa
Source organism: Glycine max
UniProt:
  • Canonical: P09186 (Residues: 1-857; Coverage: 100%)
Gene names: LOX1.3, LOX3
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
2 modified residues:
Ligand HTR 1 x HTR
Ligand HLU 1 x HLU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 111.001Å b: 136.901Å c: 61.512Å
α: 90° β: 96.24° γ: 90°
R-values:
R R work R free
0.221 0.221 0.231

Quick links

Citations

1 review citation

EPR Spectroscopic Studies of Lipoxygenases.
Gaffney BJ. (2020)
6 other articles

2 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
1 more