› 1rwd

Solution NMR

Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings

Released:
DOI: 10.2210/pdb1rwd/pdb
PDB entry 1rwd coloured by chain, front view.
PDB entry 1rwd coloured by chain, side view.
PDB entry 1rwd coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
A dipolar coupling based strategy for simultaneous resonance assignment and structure determination of protein backbones.
Tian F, Valafar H, Prestegard JH
J Am Chem Soc 123 11791-6 (2001)
PMID: 11716736

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rubredoxin Chain: A
Molecule details ›
Chain: A
Length: 53 amino acids
Theoretical weight: 5.86 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24297 (Residues: 2-54; Coverage: 98%)
Gene names: PF1282, rub
Sequence domains: Rubredoxin
Structure domains: Rubrerythrin, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: RDC directed fragment assembly
Expression system: Escherichia coli

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Citations

5 review citations

Simultaneous definition of high resolution protein structure and backbone conformational dynamics using NMR residual dipolar couplings.
Bouvignies et al. (2007)
4 more

4 mentions without citation

REDCRAFT: a tool for simultaneous characterization of protein backbone structure and motion from RDC data.
Bryson et al. (2008)
3 more