› 1rxx

X-ray diffraction
2.45Å resolution

Structure of arginine deiminase

Released:
DOI: 10.2210/pdb1rxx/pdb
PDB entry 1rxx coloured by chain, front view.
PDB entry 1rxx coloured by chain, side view.
PDB entry 1rxx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural insight into arginine degradation by arginine deiminase, an antibacterial and parasite drug target.
Galkin A, Kulakova L, Sarikaya E, Lim K, Howard A, Herzberg O
J Biol Chem 279 14001-8 (2004)
PMID: 14701825

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-citrulline + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine deiminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 421 amino acids
Theoretical weight: 47.29 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P13981 (Residues: 2-418; Coverage: 100%)
Gene names: PA5171, arcA
Sequence domains: Arginine deiminase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P41212
Unit cell:
a: 114.9Å b: 114.9Å c: 300.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.264
Expression system: Escherichia coli

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Citations

9 review citations

Dimethylarginine dimethylaminohydrolase (DDAH): expression, regulation, and function in the cardiovascular and renal systems.
Palm et al. (2007)
8 more

7 mentions without citation

PSI-BLAST-ISS: an intermediate sequence search tool for estimation of the position-specific alignment reliability.
Margelevicius et al. (2005)
6 more