› 1rzr

X-ray diffraction
2.8Å resolution

crystal structure of transcriptional regulator-phosphoprotein-DNA complex

Released:
DOI: 10.2210/pdb1rzr/pdb
PDB entry 1rzr coloured by chain, front view.
PDB entry 1rzr coloured by chain, side view.
PDB entry 1rzr coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P.
Schumacher MA, Allen GS, Diel M, Seidel G, Hillen W, Brennan RG
Cell 118 731-41 (2004)
PMID: 15369672

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114555 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Catabolite control protein A Chains: A, C, G
Molecule details ›
Chains: A, C, G
Length: 332 amino acids
Theoretical weight: 37.15 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P46828 (Residues: 1-332; Coverage: 100%)
Gene name: ccpA
Sequence domains:
Structure domains:
Catabolite control protein A Chain: D
Molecule details ›
Chain: D
Length: 332 amino acids
Theoretical weight: 37.11 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P46828 (Residues: 1-332; Coverage: 100%)
Gene name: ccpA
Sequence domains:
Structure domains:
Phosphocarrier protein HPr Chains: L, S, T, Y
Molecule details ›
Chains: L, S, T, Y
Length: 88 amino acids
Theoretical weight: 9.21 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: O69250 (Residues: 1-88; Coverage: 100%)
Gene name: ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like
5'-D(*CP*TP*GP*AP*AP*AP*GP*CP*GP*CP*TP*AP*AP*CP*AP*G)-3' Chains: E, H
Molecule details ›
Chains: E, H
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
5'-D(*CP*TP*GP*TP*TP*AP*GP*CP*GP*CP*TP*TP*TP*CP*AP*G)-3' Chains: B, R
Molecule details ›
Chains: B, R
Length: 16 nucleotides
Theoretical weight: 4.88 KDa

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand MG 2 x MG
2 modified residues:
Ligand MSE 39 x MSE
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 105.71Å b: 109.24Å c: 117.81Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.242 0.242 0.288
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Carbon catabolite repression in bacteria: many ways to make the most out of nutrients.
Görke et al. (2008)
18 more

13 mentions without citation

A novel mode of regulation of the Staphylococcus aureus catabolite control protein A (CcpA) mediated by Stk1 protein phosphorylation.
Leiba et al. (2012)
12 more