› 1s29

X-ray diffraction
1.6Å resolution

La autoantigen N-terminal domain

Released:
DOI: 10.2210/pdb1s29/pdb
PDB entry 1s29 coloured by chain, front view.
PDB entry 1s29 coloured by chain, side view.
PDB entry 1s29 coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Structure of the La motif: a winged helix domain mediates RNA binding via a conserved aromatic patch.
Dong G, Chakshusmathi G, Wolin SL, Reinisch KM
EMBO J 23 1000-7 (2004)
PMID: 14976553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA binding protein La-like protein Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.65 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NIH4 (Residues: 1-89; Coverage: 27%)
Sequence domains: La domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 32.926Å b: 53.912Å c: 53.833Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.25
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
14 more

7 mentions without citation

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
6 more