› 1s2l

X-ray diffraction
2.1Å resolution

Purine 2'deoxyribosyltransferase native structure

Released:
DOI: 10.2210/pdb1s2l/pdb
PDB entry 1s2l coloured by chain, front view.
PDB entry 1s2l coloured by chain, side view.
PDB entry 1s2l coloured by chain, top view.
Source organism: Lactobacillus helveticus
Primary publication:
Structures of purine 2'-deoxyribosyltransferase, substrate complexes, and the ribosylated enzyme intermediate at 2.0 A resolution.
Anand R, Kaminski PA, Ealick SE
Biochemistry 43 2384-93 (2004)
PMID: 14992575

Function and Biology Details

Reaction catalysed: 
2-deoxy-D-ribosyl-base(1) + base(2) = 2-deoxy-D-ribosyl-base(2) + base(1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186003 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside 2-deoxyribosyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 167 amino acids
Theoretical weight: 18.73 KDa
Source organism: Lactobacillus helveticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RLY5 (Residues: 1-167; Coverage: 100%)
Gene names: BC335_1936, GDZ32_10450, IMAU30003_01598, LH5_01102, ndtA, ntd_1, ptd
Sequence domains: Nucleoside 2-deoxyribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P43212
Unit cell:
a: 79.29Å b: 79.291Å c: 187.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.275
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biocatalytic approaches applied to the synthesis of nucleoside prodrugs.
Iglesias et al. (2015)
1 more

2 mentions without citation

Reversal of the substrate specificity of CMP N-glycosidase to dCMP.
Sikowitz et al. (2013)
1 more