› 1s4b

X-ray diffraction
2Å resolution

Crystal structure of human thimet oligopeptidase.

Released:
DOI: 10.2210/pdb1s4b/pdb
PDB entry 1s4b coloured by chain, front view.
PDB entry 1s4b coloured by chain, side view.
PDB entry 1s4b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human thimet oligopeptidase provides insight into substrate recognition, regulation, and localization.
Ray K, Hines CS, Coll-Rodriguez J, Rodgers DW
J Biol Chem 279 20480-9 (2004)
PMID: 14998993

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thimet oligopeptidase Chain: P
Molecule details ›
Chain: P
Length: 674 amino acids
Theoretical weight: 77.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52888 (Residues: 16-689; Coverage: 98%)
Gene name: THOP1
Sequence domains: Peptidase family M3
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 77.167Å b: 99.104Å c: 105.505Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.233
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure and mechanism of metallocarboxypeptidases.
Gomis-Rüth FX. (2008)
7 more

10 mentions without citation

Modeling SARS-CoV-2 spike/ACE2 protein-protein interactions for predicting the binding affinity of new spike variants for ACE2, and novel ACE2 structurally related human protein targets, for COVID-19 handling in the 3PM context.
Tragni et al. (2022)
9 more