› 1s6i

Solution NMR

Ca2+-regulatory region (CLD) from soybean calcium-dependent protein kinase-alpha (CDPK) in the presence of Ca2+ and the junction domain (JD)

Released:
DOI: 10.2210/pdb1s6i/pdb
PDB entry 1s6i coloured by chain, ensemble of 15 models, front view.
PDB entry 1s6i coloured by chain, ensemble of 15 models, side view.
PDB entry 1s6i coloured by chain, ensemble of 15 models, top view.
Source organism: Glycine max
Primary publication:
Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha.
Weljie AM, Vogel HJ
J Biol Chem 279 35494-502 (2004)
PMID: 15155727

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151246 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-dependent protein kinase SK5 Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.26 KDa
Source organism: Glycine max
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28583 (Residues: 329-470, 471-508; Coverage: 35%)
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing; molecular dynamics; matrix relaxation
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

CDPKs in immune and stress signaling.
Boudsocq et al. (2013)
7 more

2 mentions without citation

DNA origami based Au-Ag-core-shell nanoparticle dimers with single-molecule SERS sensitivity.
Prinz et al. (2016)
1 more