› 1s77

X-ray diffraction
2.69Å resolution

T7 RNAP product pyrophosphate elongation complex

Released:
DOI: 10.2210/pdb1s77/pdb
PDB entry 1s77 coloured by chain, front view.
PDB entry 1s77 coloured by chain, side view.
PDB entry 1s77 coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
The structural mechanism of translocation and helicase activity in T7 RNA polymerase.
Yin YW, Steitz TA
Cell 116 393-404 (2004)
PMID: 15016374

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114577 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chain: D
Molecule details ›
Chain: D
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*CP*GP*TP*GP*CP*GP*CP*AP*TP*TP*CP*GP*CP*CP*GP*TP*GP*TP*T)-3') Chain: T
Molecule details ›
Chain: T
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*AP*CP*GP*TP*TP*GP*CP*GP*CP*AP*CP*GP*GP*C)-3') Chain: N
Molecule details ›
Chain: N
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
RNA (5'-R(*AP*CP*AP*CP*GP*GP*CP*GP*AP*(3DA))-3') Chain: R
Molecule details ›
Chain: R
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand POP 1 x POP
1 modified residue:
Ligand 3DA 1 x 3DA

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2221
Unit cell:
a: 127.738Å b: 141.843Å c: 142.856Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.251 0.285
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

38 review citations

Making and breaking nucleic acids: two-Mg2+-ion catalysis and substrate specificity.
Yang et al. (2006)
37 more

17 mentions without citation

Computational simulation strategies for analysis of multisubunit RNA polymerases.
Wang et al. (2013)
16 more