› 1s78

X-ray diffraction
3.25Å resolution

Insights into ErbB signaling from the structure of the ErbB2-pertuzumab complex

Released:
DOI: 10.2210/pdb1s78/pdb
PDB entry 1s78 coloured by chain, front view.
PDB entry 1s78 coloured by chain, side view.
PDB entry 1s78 coloured by chain, top view.
Source organisms:
Primary publication:
Insights into ErbB signaling from the structure of the ErbB2-pertuzumab complex.
Franklin MC, Carey KD, Vajdos FF, Leahy DJ, de Vos AM, Sliwkowski MX
Cancer Cell 5 317-28 (2004)
PMID: 15093539

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-209112 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Receptor tyrosine-protein kinase erbB-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 624 amino acids
Theoretical weight: 68.79 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 23-646; Coverage: 51%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chains: C, E
Molecule details ›
Chains: C, E
Length: 214 amino acids
Theoretical weight: 23.55 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z3Y4 (Residues: 23-236; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Pertuzumab Fab heavy chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 226 amino acids
Theoretical weight: 24.16 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P43212
Unit cell:
a: 139.412Å b: 139.412Å c: 356.873Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.268
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

240 review citations

ERBB receptors and cancer: the complexity of targeted inhibitors.
Hynes et al. (2005)
239 more

60 mentions without citation

The role of HER2 in cancer therapy and targeted drug delivery.
Tai et al. (2010)
59 more