› 1s8f

X-ray diffraction
1.77Å resolution

Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II

Released:
DOI: 10.2210/pdb1s8f/pdb
PDB entry 1s8f coloured by chain, front view.
PDB entry 1s8f coloured by chain, side view.
PDB entry 1s8f coloured by chain, top view.
Source organism: Canis lupus familiaris
Primary publication:
Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II.
Wittmann JG, Rudolph MG
FEBS Lett 568 23-9 (2004)
PMID: 15196914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-150225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-9A Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.04 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P24408 (Residues: 1-175; Coverage: 87%)
Gene names: RAB9, RAB9A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

5 bound ligands:
Ligand SR 2 x SR
Ligand GDP 2 x GDP
Ligand BEZ 4 x BEZ
Ligand MG 1 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: I41
Unit cell:
a: 98.245Å b: 98.245Å c: 79.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.221
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural clues to Rab GTPase functional diversity.
Pfeffer SR. (2005)
12 other articles

4 mentions without citation

Structural mechanisms for regulation of membrane traffic by rab GTPases.
Lee et al. (2009)
3 more