› 1sb7

X-ray diffraction
2.2Å resolution

Crystal structure of the E.coli pseudouridine synthase TruD

Released:
DOI: 10.2210/pdb1sb7/pdb
PDB entry 1sb7 coloured by chain, front view.
PDB entry 1sb7 coloured by chain, side view.
PDB entry 1sb7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the highly divergent pseudouridine synthase TruD reveals a circular permutation of a conserved fold.
Hoang C, Ferre-D'Amare AR
RNA 10 1026-33 (2004)
PMID: 15208439

Function and Biology Details

Reaction catalysed: 
tRNA uridine(13) = tRNA pseudouridine(13)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA pseudouridine synthase D Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 41.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57261 (Residues: 1-349; Coverage: 100%)
Gene names: JW2715, b2745, truD, ygbO
Sequence domains: tRNA pseudouridine synthase D (TruD)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 63.665Å b: 108.78Å c: 111.855Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Pseudouridine synthases.
Hamma et al. (2006)
6 more

1 mention without citation

Crystal structure of the highly divergent pseudouridine synthase TruD reveals a circular permutation of a conserved fold.
Hoang et al. (2004)