› 1sbq

X-ray diffraction
2.2Å resolution

Crystal Structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae at 2.2 resolution

Released:
DOI: 10.2210/pdb1sbq/pdb
PDB entry 1sbq coloured by chain, front view.
PDB entry 1sbq coloured by chain, side view.
PDB entry 1sbq coloured by chain, top view.
Source organism: Mycoplasma pneumoniae
Primary publication:
Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2 A resolution.
Chen S, Shin DH, Pufan R, Kim R, Kim SH
Proteins 56 839-43 (2004)
PMID: 15281135

Function and Biology Details

Reaction catalysed: 
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-formyltetrahydrofolate cyclo-ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 22.27 KDa
Source organism: Mycoplasma pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P75430 (Residues: 1-164; Coverage: 100%)
Gene names: H91_orf164, MP488, MPN_348
Sequence domains: 5-formyltetrahydrofolate cyclo-ligase family
Structure domains: NagB/RpiA/CoA transferase-like

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 42.315Å b: 80.746Å c: 132.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.239 0.27
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Diversification of catalytic activities and ligand interactions in the protein fold shared by the sugar isomerases, eIF2B, DeoR transcription factors, acyl-CoA transferases and methenyltetrahydrofolate synthetase.
Anantharaman et al. (2006)
6 more

2 mentions without citation

Conformational changes in redox pairs of protein structures.
Fan et al. (2009)
1 more