› 1sd1

X-ray diffraction
2.03Å resolution

STRUCTURE OF HUMAN 5'-DEOXY-5'-METHYLTHIOADENOSINE PHOSPHORYLASE COMPLEXED WITH FORMYCIN A

Released:
DOI: 10.2210/pdb1sd1/pdb
PDB entry 1sd1 coloured by chain, front view.
PDB entry 1sd1 coloured by chain, side view.
PDB entry 1sd1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural comparison of MTA phosphorylase and MTA/AdoHcy nucleosidase explains substrate preferences and identifies regions exploitable for inhibitor design.
Lee JE, Settembre EC, Cornell KA, Riscoe MK, Sufrin JR, Ealick SE, Howell PL
Biochemistry 43 5159-69 (2004)
PMID: 15122881

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 31.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13126 (Residues: 1-283; Coverage: 100%)
Gene names: MSAP, MTAP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand FMC 1 x FMC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P321
Unit cell:
a: 121Å b: 121Å c: 44.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.18 0.198
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Making 'sense' of metabolism: autoinducer-2, LuxS and pathogenic bacteria.
Vendeville et al. (2005)
2 more

1 mention without citation

Interference With Quorum-Sensing Signal Biosynthesis as a Promising Therapeutic Strategy Against Multidrug-Resistant Pathogens.
Fleitas Martínez et al. (2018)