› 1se8

X-ray diffraction
1.8Å resolution

Structure of single-stranded DNA-binding protein (SSB) from D. radiodurans

Released:
DOI: 10.2210/pdb1se8/pdb
PDB entry 1se8 coloured by chain, front view.
PDB entry 1se8 coloured by chain, side view.
PDB entry 1se8 coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
Crystal structure of the Deinococcus radiodurans single-stranded DNA-binding protein suggests a mechanism for coping with DNA damage.
Bernstein DA, Eggington JM, Killoran MP, Misic AM, Cox MM, Keck JL
Proc Natl Acad Sci U S A 101 8575-80 (2004)
PMID: 15159541

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 33.18 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RY51 (Residues: 1-301; Coverage: 100%)
Gene names: DR_0099, ssb
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2
Unit cell:
a: 91.347Å b: 63.476Å c: 54.907Å
α: 90° β: 106.27° γ: 90°
R-values:
R R work R free
0.233 0.217 0.232
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Deinococcus radiodurans - the consummate survivor.
Cox et al. (2005)
14 more

5 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
4 more