› 1sff

X-ray diffraction
1.9Å resolution

Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate

Released:
DOI: 10.2210/pdb1sff/pdb
PDB entry 1sff coloured by chain, front view.
PDB entry 1sff coloured by chain, side view.
PDB entry 1sff coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of unbound and aminooxyacetate-bound Escherichia coli gamma-aminobutyrate aminotransferase.
Liu W, Peterson PE, Carter RJ, Zhou X, Langston JA, Fisher AJ, Toney MD
Biochemistry 43 10896-905 (2004)
PMID: 15323550

Function and Biology Details

Reactions catalysed: 
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
5-aminopentanoate + 2-oxoglutarate = 5-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-aminobutyrate aminotransferase GabT Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 426 amino acids
Theoretical weight: 45.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P22256 (Residues: 1-426; Coverage: 100%)
Gene names: JW2637, b2662, gabT
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 11 x SO4
Ligand EDO 14 x EDO
Ligand IK2 4 x IK2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3221
Unit cell:
a: 108.15Å b: 108.15Å c: 301.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.159 0.186
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Controlling reaction specificity in pyridoxal phosphate enzymes.
Toney MD. (2011)
3 more

7 mentions without citation

Evolutionary origin and functional diversification of aminotransferases.
Koper et al. (2022)
6 more