› 1sgt

X-ray diffraction
1.7Å resolution

REFINED CRYSTAL STRUCTURE OF STREPTOMYCES GRISEUS TRYPSIN AT 1.7 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1sgt/pdb
PDB entry 1sgt coloured by chain, front view.
PDB entry 1sgt coloured by chain, side view.
PDB entry 1sgt coloured by chain, top view.
Source organism: Streptomyces griseus
Primary publication:
Refined crystal structure of Streptomyces griseus trypsin at 1.7 A resolution.
Read RJ, James MN
J Mol Biol 200 523-51 (1988)
PMID: 3135412

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.08 KDa
Source organism: Streptomyces griseus
Expression system: Not provided
UniProt:
  • Canonical: P00775 (Residues: 37-259; Coverage: 98%)
Gene name: sprT
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 72.29Å b: 50.98Å c: 120.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 not available not available
Expression system: Not provided

Quick links

Citations

5 review citations

Structural basis of substrate specificity in the serine proteases.
Perona et al. (1995)
4 more

17 mentions without citation

The limits of protein sequence comparison?
Pearson et al. (2005)
16 more