› 1si2

X-ray diffraction
2.6Å resolution

Crystal structure of the PAZ domain of human eIF2c1 in complex with a 9-mer siRNA-like duplex of deoxynucleotide overhang

Released:
DOI: 10.2210/pdb1si2/pdb
PDB entry 1si2 coloured by chain, front view.
PDB entry 1si2 coloured by chain, side view.
PDB entry 1si2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain.
Ma JB, Ye K, Patel DJ
Nature 429 318-322 (2004)
PMID: 15152257

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113090 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Protein argonaute-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 17.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UL18 (Residues: 225-369; Coverage: 17%)
Gene names: AGO1, EIF2C1
Sequence domains: PAZ domain
Structure domains: paz domain
5'-R(*CP*GP*UP*GP*AP*CP*U)-D(P*CP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.79 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P64
Unit cell:
a: 100.051Å b: 100.051Å c: 34.438Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.221 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

118 review citations

Biogenesis of small RNAs in animals.
Kim et al. (2009)
117 more

8 mentions without citation

Therapeutic potential of chemically modified siRNA: Recent trends.
Selvam et al. (2017)
7 more