› 1sii

X-ray diffraction
1.7Å resolution

AGAO in covalent complex with the inhibitor NOBA ("4-(2-naphthyloxy)-2-butyn-1-amine")

Released:
DOI: 10.2210/pdb1sii/pdb
PDB entry 1sii coloured by chain, front view.
PDB entry 1sii coloured by chain, side view.
PDB entry 1sii coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Differential inhibition of six copper amine oxidases by a family of 4-(aryloxy)-2-butynamines: evidence for a new mode of inactivation.
O'Connell KM, Langley DB, Shepard EM, Duff AP, Jeon HB, Sun G, Freeman HC, Guss JM, Sayre LM, Dooley DM
Biochemistry 43 10965-78 (2004)
PMID: 15323556

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chain: A
Molecule details ›
Chain: A
Length: 646 amino acids
Theoretical weight: 71.98 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46881 (Residues: 3-638; Coverage: 100%)
Sequence domains: Copper amine oxidase, enzyme domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CU 1 x CU
Ligand NA 1 x NA
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand NBQ 1 x NBQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 158.06Å b: 62.619Å c: 91.99Å
α: 90° β: 112.11° γ: 90°
R-values:
R R work R free
0.159 0.157 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Copper active sites in biology.
Solomon et al. (2014)
1 more

1 mention without citation

Enantiomer-specific binding of ruthenium(II) molecular wires by the amine oxidase of Arthrobacter globiformis.
Langley et al. (2008)