› 1siz

X-ray diffraction
2.25Å resolution

Crystal structure of the [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1siz/pdb
PDB entry 1siz coloured by chain, front view.
PDB entry 1siz coloured by chain, side view.
PDB entry 1siz coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.
Nielsen MS, Harris P, Ooi BL, Christensen HE
Biochemistry 43 5188-94 (2004)
PMID: 15122884

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin Chains: A, C
Molecule details ›
Chains: A, C
Length: 66 amino acids
Theoretical weight: 7.17 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29603 (Residues: 2-67; Coverage: 99%)
Gene names: PF1909, fdxA
Sequence domains: 4Fe-4S single cluster domain of Ferredoxin I
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand 3CO 4 x 3CO
Ligand F3S 2 x F3S
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 47.447Å b: 51.637Å c: 54.549Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.289
Expression system: Escherichia coli

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Citations

2 review citations

Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily.
Munro et al. (2007)
1 more