› 1sj1

X-ray diffraction
1.5Å resolution

The 1.5 A Resolution Crystal Structure of [Fe3S4]-Ferredoxin from the hyperthermophilic Archaeon Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1sj1/pdb
PDB entry 1sj1 coloured by chain, front view.
PDB entry 1sj1 coloured by chain, side view.
PDB entry 1sj1 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.
Nielsen MS, Harris P, Ooi BL, Christensen HE
Biochemistry 43 5188-94 (2004)
PMID: 15122884

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.17 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29603 (Residues: 2-67; Coverage: 99%)
Gene names: PF1909, fdxA
Sequence domains: 4Fe-4S single cluster domain of Ferredoxin I
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand NCO 4 x NCO
Ligand F3S 2 x F3S
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 31.23Å b: 47.511Å c: 52.029Å
α: 90° β: 103.86° γ: 90°
R-values:
R R work R free
0.195 0.194 0.223
Expression system: Escherichia coli

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Citations

2 review citations

Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily.
Munro et al. (2007)
1 more

5 mentions without citation

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS).
Hura et al. (2009)
4 more