› 1sj7

X-ray diffraction
2.5Å resolution

Crystal Structure of Talin Rod 482-655

Released:
DOI: 10.2210/pdb1sj7/pdb
PDB entry 1sj7 coloured by chain, front view.
PDB entry 1sj7 coloured by chain, side view.
PDB entry 1sj7 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle.
Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J
EMBO J 23 2942-51 (2004)
PMID: 15272303

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-150641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Talin-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 174 amino acids
Theoretical weight: 17.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26039 (Residues: 482-655; Coverage: 7%)
Gene names: Tln, Tln1
Sequence domains: Talin, middle domain
Structure domains: Talin, central domain

Ligands and Environments

1 bound ligand:
Ligand PT 7 x PT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 95.662Å b: 95.662Å c: 115.215Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.241 0.286
Expression system: Escherichia coli BL21

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Citations

31 review citations

Focal adhesion kinase: in command and control of cell motility.
Mitra et al. (2005)
30 more

4 mentions without citation

Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.
Johnson et al. (2007)
3 more