› 1sjs

X-ray diffraction
2.42Å resolution

ACCESS TO PHOSPHORYLATION IN ISOCITRATE DEHYDROGENASE MAY OCCUR BY DOMAIN SHIFTING

Released:
DOI: 10.2210/pdb1sjs/pdb
PDB entry 1sjs coloured by chain, front view.
PDB entry 1sjs coloured by chain, side view.
PDB entry 1sjs coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Access to phosphorylation in isocitrate dehydrogenase may occur by domain shifting.
Finer-Moore J, Tsutakawa SE, Cherbavaz DR, LaPorte DC, Koshland DE, Stroud RM
Biochemistry 36 13890-6 (1997)
PMID: 9374867

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 45.81 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P08200 (Residues: 1-416; Coverage: 100%)
Gene names: JW1122, b1136, icd, icdA, icdE
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 124.5Å b: 77.5Å c: 92.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.23
Expression system: Not provided

Quick links

Citations

26 citation in other articles

Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity.
Xu et al. (2004)
25 more

2 mentions without citation

Induced fit and the catalytic mechanism of isocitrate dehydrogenase.
Gonçalves et al. (2012)
1 more