› 1snf

X-ray diffraction
1.85Å resolution

MYCOBACTERIUM TUBERCULOSIS DUTPASE COMPLEXED WITH MAGNESIUM AND DEOXYURIDINE 5'-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1snf/pdb
PDB entry 1snf coloured by chain, front view.
PDB entry 1snf coloured by chain, side view.
PDB entry 1snf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of the Mycobacterium tuberculosis dUTPase: insights into the catalytic mechanism.
Chan S, Segelke B, Lekin T, Krupka H, Cho US, Kim MY, So M, Kim CY, Naranjo CM, Rogers YC, Park MS, Waldo GS, Pashkov I, Cascio D, Perry JL, Sawaya MR
J Mol Biol 341 503-17 (2004)
PMID: 15276840

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyuridine 5'-triphosphate nucleotidohydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 174 amino acids
Theoretical weight: 17.99 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNS5 (Residues: 1-154; Coverage: 100%)
Gene names: MTCY05A6.18c, Rv2697c, dut
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand NO3 3 x NO3
Ligand UMP 3 x UMP
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 58.228Å b: 77.496Å c: 93.949Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.169 0.205
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The phage-related chromosomal islands of Gram-positive bacteria.
Novick et al. (2010)
5 more

2 mentions without citation

Presenting your structures: the CCP4mg molecular-graphics software.
McNicholas et al. (2011)
1 more