› 1so8

X-ray diffraction
2.3Å resolution

Abeta-bound human ABAD structure [also known as 3-hydroxyacyl-CoA dehydrogenase type II (Type II HADH), Endoplasmic reticulum-associated amyloid beta-peptide binding protein (ERAB)]

Released:
DOI: 10.2210/pdb1so8/pdb
PDB entry 1so8 coloured by chain, front view.
PDB entry 1so8 coloured by chain, side view.
PDB entry 1so8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
ABAD directly links Abeta to mitochondrial toxicity in Alzheimer's disease.
Lustbader JW, Cirilli M, Lin C, Xu HW, Takuma K, Wang N, Caspersen C, Chen X, Pollak S, Chaney M, Trinchese F, Liu S, Gunn-Moore F, Lue LF, Walker DG, Kuppusamy P, Zewier ZL, Arancio O, Stern D, Yan SS, Wu H
Science 304 448-52 (2004)
PMID: 15087549

Function and Biology Details

Reactions catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methylacetoacetyl-CoA + NADH
Testosterone + NAD(+) = androstenedione + NADH
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-189254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyacyl-CoA dehydrogenase type-2 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 26.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99714 (Residues: 1-261; Coverage: 100%)
Gene names: ERAB, HADH2, HSD17B10, MRPP2, SCHAD, SDR5C1, XH98G2
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P432
Unit cell:
a: 130Å b: 130Å c: 130Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.261
Expression system: Escherichia coli BL21

Quick links

Citations

372 review citations

Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases.
Lin et al. (2006)
371 more

5 mentions without citation

Infantile Neurodegeneration Results from Mutants of 17β-Hydroxysteroid Dehydrogenase Type 10 Rather Than Aβ-Binding Alcohol Dehydrogenase.
He et al. (2023)
4 more