› 1sq9

X-ray diffraction
1.9Å resolution

Structure of Ski8p, a WD repeat protein involved in mRNA degradation and meiotic recombination

Released:
DOI: 10.2210/pdb1sq9/pdb
PDB entry 1sq9 coloured by chain, front view.
PDB entry 1sq9 coloured by chain, side view.
PDB entry 1sq9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure.
Madrona AY, Wilson DK
Protein Sci 13 1557-65 (2004)
PMID: 15152089

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169764 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral protein SKI8 Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 44.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02793 (Residues: 1-397; Coverage: 100%)
Gene names: REC103, SKI8, YGL213C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 65.54Å b: 67.64Å c: 80.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.246
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Ski2-like RNA helicase structures: common themes and complex assemblies.
Johnson et al. (2013)
2 more

6 mentions without citation

Bioinformatic analyses implicate the collaborating meiotic crossover/chiasma proteins Zip2, Zip3, and Spo22/Zip4 in ubiquitin labeling.
Perry et al. (2005)
5 more