1ssb

X-ray diffraction
2Å resolution

A STRUCTURAL INVESTIGATION OF CATALYTICALLY MODIFIED F12OL AND F12OY SEMISYNTHETIC RIBONUCLEASES

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-158391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.05 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-144; Coverage: 95%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
Ribonuclease pancreatic Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.52 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 137-150; Coverage: 11%)
Gene names: RNASE1, RNS1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 68.15Å b: 68.15Å c: 65.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 not available
Expression system: Not provided