› 1st6

X-ray diffraction
3.1Å resolution

Crystal structure of a cytoskeletal protein

Released:
DOI: 10.2210/pdb1st6/pdb
PDB entry 1st6 coloured by chain, front view.
PDB entry 1st6 coloured by chain, side view.
PDB entry 1st6 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis for vinculin activation at sites of cell adhesion.
Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW, Liddington RC
Nature 430 583-6 (2004)
PMID: 15195105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 1069 amino acids
Theoretical weight: 117.51 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12003 (Residues: 1-1135; Coverage: 94%)
Gene names: VCL, VINC1
Sequence domains: Vinculin family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2221
Unit cell:
a: 56.02Å b: 126.947Å c: 351.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.318 0.316 0.357
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

44 review citations

Local force and geometry sensing regulate cell functions.
Vogel et al. (2006)
43 more

19 mentions without citation

An autoinhibited structure of α-catenin and its implications for vinculin recruitment to adherens junctions.
Ishiyama et al. (2013)
18 more